This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCEP0057905
PrimePCR™ PreAmp for SYBR® Green Assay: EPHA10, Human
PrimePCR™ Template for SYBR® Green Assay: EPHA10, Human
Ephrin receptors the largest subfamily of receptor tyrosine kinases (RTKs) and their ephrin ligands are important mediators of cell-cell communication regulating cell attachment shape and mobility in neuronal and epithelial cells (Aasheim et al. 2005 [PubMed 15777695]). See MIM 179610 for additional background on Eph receptors and ephrins.[supplied by OMIM Mar 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.