PrimePCR™ SYBR® Green Assay: EPHA10, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0057905

List Price:    $130.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0056800
Assay Design:   exonic
Chromosome Location:   1:38220041-38227247question
Amplicon Length:   143
Splice Variants Targeted:   ENST00000216044 ENST00000572888 ENST00000342782 ENST00000373048 ENST00000319637 ENST00000427468 ENST00000397360 ENST00000394729 ENST00000330452 ENST00000344204 ENST00000344135 ENST00000537187 ENST00000259808 ENST00000541791 ENST00000380409 ENST00000599661

Gene Information

Ephrin receptors the largest subfamily of receptor tyrosine kinases (RTKs) and their ephrin ligands are important mediators of cell-cell communication regulating cell attachment shape and mobility in neuronal and epithelial cells (Aasheim et al. 2005 [PubMed 15777695]). See MIM 179610 for additional background on Eph receptors and ephrins.[supplied by OMIM Mar 2008]

Gene Symbol:   EPHA10
Gene Name:   EPH receptor A10
Aliases:   FLJ16103, FLJ33655, MGC43817
RefSeq:   NC_000001.10 NT_032977.9
Ensembl:   ENSG00000183317
Entrez:   284656
UniGene:   Hs.129435
Chromosome Mapping:   1p34.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 35.990000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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