This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0057888
PrimePCR™ PreAmp for SYBR® Green Assay: ZIC4, Human
PrimePCR™ Template for SYBR® Green Assay: ZIC4, Human
This gene encodes a member of the ZIC family of C2H2-type zinc finger proteins. Members of this family are important during development and have been associated with X-linked visceral heterotaxy and holoprosencephaly type 5. This gene is closely linked to the gene encoding zinc finger protein of the cerebellum 1 a related family member on chromosome 3. Heterozygous deletion of these linked genes is involved in Dandy-Walker malformation which is a congenital cerebellar malformation. Multiple transcript variants have been identified for this gene. [provided by RefSeq Dec 2009]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.