PrimePCR™ SYBR® Green Assay: BTN2A2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0057885

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: BTN2A2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: BTN2A2, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0056780
Assay Design:   exonic
Chromosome Location:   6:26390245-26390347question
Amplicon Length:   73
Splice Variants Targeted:   ENST00000398988 ENST00000341948 ENST00000354293 ENST00000359032 ENST00000215838 ENST00000407817 ENST00000361926 ENST00000421050 ENST00000341901 ENST00000261405 ENST00000392561 ENST00000373823 ENST00000373818 ENST00000545652 ENST00000436847 ENST00000394353 ENST00000449733 ENST00000412819 ENST00000341272 ENST00000373808 ENST00000469230 ENST00000490025 ENST00000356709 ENST00000352867 ENST00000482536 ENST00000432533 ENST00000482842 ENST00000416795 ENST00000483410 ENST00000392313 ENST00000262815 ENST

Gene Information

Butyrophilin is the major protein associated with fat droplets in the milk. This gene is a member of the BTN2 subfamily of genes which encode proteins belonging to the butyrophilin protein family. The gene is located in a cluster on chromosome 6 consisting of seven genes belonging to the expanding B7/butyrophilin-like group a subset of the immunoglobulin gene superfamily. The encoded protein is a type I receptor glycoprotein involved in lipid fatty-acid and sterol metabolism. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Oct 2010]

Gene Symbol:   BTN2A2
Gene Name:   butyrophilin, subfamily 2, member A2
Aliases:   BT2.2, BTF2, FLJ41908
RefSeq:   NC_000006.11 NT_007592.15
Ensembl:   ENSG00000124508
Entrez:   10385
Chromosome Mapping:   6p22.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998100
y-intercept 36.600000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download