PrimePCR™ SYBR® Green Assay: SEC14L2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0057882

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: SEC14L2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: SEC14L2, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0056777
Assay Design:   exonic
Chromosome Location:   22:30803459-30803548question
Amplicon Length:   60
Splice Variants Targeted:   ENST00000312932 ENST00000428195 ENST00000403484 ENST00000416523 ENST00000405717 ENST00000429917 ENST00000399054 ENST00000347364 ENST00000526627 ENST00000309765 ENST00000377989

Gene Information

This gene encodes a cytosolic protein which belongs to a family of lipid-binding proteins including Sec14p alpha-tocopherol transfer protein and cellular retinol-binding protein. The encoded protein stimulates squalene monooxygenase which is a downstream enzyme in the cholesterol biosynthetic pathway. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq Oct 2008]

Gene Symbol:   SEC14L2
Gene Name:   SEC14-like 2 (S. cerevisiae)
Aliases:   C22orf6, KIAA1186, KIAA1658, MGC65053, SPF, TAP, TAP1
RefSeq:   NC_000022.10 NT_011520.12
Ensembl:   ENSG00000100003
Entrez:   23541
Chromosome Mapping:   22q12.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999000
y-intercept 36.650000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download