PrimePCR™ SYBR® Green Assay: CGB5, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0057873

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: CGB5, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: CGB5, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0056768
Assay Design:   exonic
Chromosome Location:   19:49547263-49547395question
Amplicon Length:   103
Splice Variants Targeted:   ENST00000357383 ENST00000301408 ENST00000448456 ENST00000355414 ENST00000596965 ENST00000597853 ENST00000377280 ENST00000356213 ENST00000469083 ENST00000373109 ENST00000317376 ENST00000536168 ENST00000410011 ENST00000391748 ENST00000314446 ENST00000391749 ENST00000434421 ENST00000251390 ENST00000391745 ENST00000335154 ENST00000550488 ENST00000352151 ENST00000439534 ENST00000251377 ENST00000251376 ENST00000391737 ENST00000391738 ENST00000396331 ENST00000396327 ENST00000396332 ENST00000427581 ENST

Gene Information

This gene is a member of the glycoprotein hormone beta chain family and encodes the beta 5 subunit of chorionic gonadotropin (CG). Glycoprotein hormones are heterodimers consisting of a common alpha subunit and an unique beta subunit which confers biological specificity. CG is produced by the trophoblastic cells of the placenta and stimulates the ovaries to synthesize the steroids that are essential for the maintenance of pregnancy. The beta subunit of CG is encoded by 6 genes which are arranged in tandem and inverted pairs on chromosome 19q13.3 and contiguous with the luteinizing hormone beta subunit gene. [provided by RefSeq Jul 2008]

Gene Symbol:   CGB5
Gene Name:   chorionic gonadotropin, beta polypeptide 5
Aliases:   HCG, MGC119822
RefSeq:   NC_000019.9 NG_000019.3 NT_011109.16
Ensembl:   ENSG00000189052
Entrez:   93659
Chromosome Mapping:   19q13.32

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 35.900000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download