PrimePCR™ SYBR® Green Assay: NOXO1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0057866

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0056761
Assay Design:   exonic
Chromosome Location:   16:2031174-2031316question
Amplicon Length:   113
Splice Variants Targeted:   ENST00000448645 ENST00000428611 ENST00000371521 ENST00000344894 ENST00000427047 ENST00000449208 ENST00000446013 ENST00000426996 ENST00000413055 ENST00000456988 ENST00000416714 ENST00000449742 ENST00000376704 ENST00000369888 ENST00000450537 ENST00000217130 ENST00000417411 ENST00000451727 ENST00000354038 ENST00000259941 ENST00000431258 ENST00000447465 ENST00000568546 ENST00000436083 ENST00000356987 ENST00000432564 ENST00000356120 ENST00000354249 ENST00000567471 ENST00000303383

Gene Information

NADPH oxidases (NOXs) catalyze the transfer of electrons from NADPH to molecular oxygen to generate reactive oxygen species (ROS). NOX organizers such as NOXO1 target NOX activators (see NOXA1; MIM 611255) to NOX and also target NOX to different subcellular compartments (Opitz et al. 2007 [PubMed 17189823]).[supplied by OMIM Mar 2008]

Gene Symbol:   NOXO1
Gene Name:   NADPH oxidase organizer 1
Aliases:   MGC20258, P41NOX, P41NOXA, P41NOXB, P41NOXC, SH3PXD5, SNX28
RefSeq:   NC_000016.9 NG_016288.1 NT_010393.16
Ensembl:   ENSG00000196408
Entrez:   124056
UniGene:   Hs.191762
Chromosome Mapping:   16p13.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 36.970000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download