This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCEP0057779
PrimePCR™ PreAmp for SYBR® Green Assay: CD276, Human
PrimePCR™ Template for SYBR® Green Assay: CD276, Human
The protein encoded by this gene belongs to the immunoglobulin superfamily and thought to participate in the regulation of T-cell-mediated immune response. Studies show that while the transcript of this gene is ubiquitously expressed in normal tissues and solid tumors the protein is preferentially expressed only in tumor tissues. Additionally it was observed that the 3' UTR of this transcript contains a target site for miR29 microRNA and there is an inverse correlation between the expression of this protein and miR29 levels suggesting regulation of expression of this gene product by miR29. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Sep 2011]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.