This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCEP0057730
PrimePCR™ PreAmp for SYBR® Green Assay: PLS1, Human
PrimePCR™ Template for SYBR® Green Assay: PLS1, Human
Plastins are a family of actin-binding proteins that are conserved throughout eukaryote evolution and expressed in most tissues of higher eukaryotes. In humans two ubiquitous plastin isoforms (L and T) have been identified. The protein encoded by this gene is a third distinct plastin isoform which is specifically expressed at high levels in the small intestine. Alternatively spliced transcript variants varying in the 5' UTR but encoding the same protein have been found for this gene. A pseudogene of this gene is found on chromosome 11.[provided by RefSeq Feb 2010]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.