PrimePCR™ SYBR® Green Assay: PPP1R13L, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0057704

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: PPP1R13L, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: PPP1R13L, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0056599
Assay Design:   exonic
Chromosome Location:   19:45883384-45883481question
Amplicon Length:   68
Splice Variants Targeted:   ENST00000360957 ENST00000591986 ENST00000418234

Gene Information

IASPP is one of the most evolutionarily conserved inhibitors of p53 (TP53; MIM 191170) whereas ASPP1 (MIM 606455) and ASPP2 (MIM 602143) are activators of p53.[supplied by OMIM Mar 2008]

Gene Symbol:   PPP1R13L
Gene Name:   protein phosphatase 1, regulatory (inhibitor) subunit 13 like
Aliases:   IASPP, NKIP1, RAI, RAI4
RefSeq:   NC_000019.9 NG_015839.1 NT_011109.16
Ensembl:   ENSG00000104881
Entrez:   10848
Chromosome Mapping:   19q13.32

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998600
y-intercept 35.710000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download