PrimePCR™ SYBR® Green Assay: SPHK1, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0057678

List Price:    $130.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0056573
Assay Design:   exonic
Chromosome Location:   17:74383045-74383194question
Amplicon Length:   120
Splice Variants Targeted:   ENST00000545180 ENST00000592299 ENST00000590959 ENST00000323374 ENST00000392496

Gene Information

The protein encoded by this gene catalyzes the phosphorylation of sphingosine to form sphingosine-1-phosphate (S1P) a lipid mediator with both intra- and extracellular functions. Intracellularly S1P regulates proliferation and survival and extracellularly it is a ligand for cell surface G protein-coupled receptors. This protein and its product S1P play a key role in TNF-alpha signaling and the NF-kappa-B activation pathway important in inflammatory antiapoptotic and immune processes. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Sep 2011]

Gene Symbol:   SPHK1
Gene Name:   sphingosine kinase 1
Aliases:   SPHK
RefSeq:   NC_000017.10 NT_010783.15
Ensembl:   ENSG00000176170
Entrez:   8877
UniGene:   Hs.68061
Chromosome Mapping:   17q25.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 36.230000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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