This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCEP0057615
PrimePCR™ PreAmp for SYBR® Green Assay: PPP1R12B, Human
PrimePCR™ Template for SYBR® Green Assay: PPP1R12B, Human
Myosin phosphatase is a protein complex comprised of three subunits: a catalytic subunit (PP1c-delta protein phosphatase 1 catalytic subunit delta) a large regulatory subunit (MYPT myosin phosphatase target) and small regulatory subunit (sm-M20). Two isoforms of MYPT have been isolated--MYPT1 and MYPT2 the first of which is widely expressed and the second of which may be specific to heart skeletal muscle and brain. Each of the MYPT isoforms functions to bind PP1c-delta and increase phosphatase activity. This locus encodes both MYTP2 and M20. Alternatively spliced transcript variants encoding different isoforms have been identified. Related pseudogenes have been defined on the Y chromosome. [provided by RefSeq Oct 2011]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.