PrimePCR™ SYBR® Green Assay: ZNF573, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0057557

List Price:    $130.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0056452
Assay Design:   exonic
Chromosome Location:   19:38260732-38262294question
Amplicon Length:   67
Splice Variants Targeted:   ENST00000274963 ENST00000304338 ENST00000553661 ENST00000556470 ENST00000324461 ENST00000588967 ENST00000593164 ENST00000310738 ENST00000587539 ENST00000324394 ENST00000591790 ENST00000504904 ENST00000292069 ENST00000587555 ENST00000589946 ENST00000342634 ENST00000373191 ENST00000246314 ENST00000355202 ENST00000395835 ENST00000591664 ENST00000590414 ENST00000392138 ENST00000378445 ENST00000588218 ENST00000536220 ENST00000339503 ENST00000443917 ENST00000598744 ENST00000282286 ENST00000391705 ENST

Gene Information

Description Not Available

Gene Symbol:   ZNF573
Gene Name:   zinc finger protein 573
Aliases:   FLJ30921
RefSeq:   NC_000019.9 NT_011109.16
Ensembl:   ENSG00000189144
Entrez:   126231
UniGene:   Hs.531262
Chromosome Mapping:   19q13.12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 36.310000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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