PrimePCR™ SYBR® Green Assay: YIF1A, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0057551

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: YIF1A, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: YIF1A, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0056446
Assay Design:   exonic
Chromosome Location:   11:66055283-66055386question
Amplicon Length:   74
Splice Variants Targeted:   ENST00000430215 ENST00000233838 ENST00000582783 ENST00000392035 ENST00000217320 ENST00000436941 ENST00000359461 ENST00000376901 ENST00000376904 ENST00000431556 ENST00000528575 ENST00000222573 ENST00000537992 ENST00000379902 ENST00000314276 ENST00000314250 ENST00000546602 ENST00000552570 ENST00000549700 ENST00000373324 ENST00000323301 ENST00000300417 ENST00000477853 ENST00000375199 ENST00000375208

Gene Information

Description Not Available

Gene Symbol:   YIF1A
Gene Name:   Yip1 interacting factor homolog A (S. cerevisiae)
Aliases:   54TM, FinGER7, YIF1, YIF1P
RefSeq:   NC_000011.9 NT_167190.1
Ensembl:   ENSG00000174851
Entrez:   10897
Chromosome Mapping:   11q13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 36.270000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download