PrimePCR™ SYBR® Green Assay: SPNS1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0057549

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: SPNS1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: SPNS1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0056444
Assay Design:   exonic
Chromosome Location:   16:28986697-28989239question
Amplicon Length:   64
Splice Variants Targeted:   ENST00000565975 ENST00000311008 ENST00000323081 ENST00000334536 ENST00000566059 ENST00000564476 ENST00000568388 ENST00000567771 ENST00000335712 ENST00000358510 ENST00000245407 ENST00000585807 ENST00000341426 ENST00000341991 ENST00000378625 ENST00000469045 ENST00000344463 ENST00000342348 ENST00000452271 ENST00000518157 ENST00000330953 ENST00000427697

Gene Information

Description Not Available

Gene Symbol:   SPNS1
Gene Name:   spinster homolog 1 (Drosophila)
Aliases:   FLJ38358, HSpin1, LAT, PP2030, SPIN1, SPINL, nrs
RefSeq:   NC_000016.9 NT_010393.16
Ensembl:   ENSG00000169682
Entrez:   83985
Chromosome Mapping:   16p11.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998700
y-intercept 35.800000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download