PrimePCR™ SYBR® Green Assay: PACSIN3, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0057546

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: PACSIN3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: PACSIN3, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0056441
Assay Design:   exonic
Chromosome Location:   11:47202063-47202152question
Amplicon Length:   60
Splice Variants Targeted:   ENST00000592696 ENST00000400720 ENST00000430208 ENST00000379441 ENST00000551812 ENST00000549884 ENST00000179765 ENST00000288439 ENST00000562273 ENST00000561937 ENST00000563713 ENST00000566505 ENST00000567429 ENST00000570171 ENST00000565093 ENST00000437160 ENST00000417993 ENST00000298838 ENST00000528462 ENST00000530513 ENST00000528201 ENST00000531226 ENST00000525725 ENST00000539589 ENST00000341640 ENST00000463946 ENST00000338516 ENST00000431123 ENST00000437383 ENST00000392830 ENST00000240050 ENST

Gene Information

This gene is a member of the protein kinase C and casein kinase substrate in neurons family. The encoded protein is involved in linking the actin cytoskeleton with vesicle formation. Alternative splicing results in multiple transcript variants. [provided by RefSeq May 2010]

Gene Symbol:   PACSIN3
Gene Name:   protein kinase C and casein kinase substrate in neurons 3
Aliases:   FLJ17861, SDPIII
RefSeq:   NC_000011.9 NT_009237.18
Ensembl:   ENSG00000165912
Entrez:   29763
Chromosome Mapping:   11p12-p11.12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.970000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download