This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0057545
PrimePCR™ PreAmp for SYBR® Green Assay: ANAPC10, Human
PrimePCR™ Template for SYBR® Green Assay: ANAPC10, Human
ANAPC10 is a core subunit of the anaphase-promoting complex (APC) or cyclosome a ubiquitin protein ligase that is essential for progression through the cell cycle. APC initiates sister chromatid separation by ubiquitinating the anaphase inhibitor securin (PTTG1; MIM 604147) and triggers exit from mitosis by ubiquitinating cyclin B (CCNB1; MIM 123836) the activating subunit of cyclin-dependent kinase-1 (CDK1; MIM 116940) (summary by Wendt et al. 2001 [PubMed 11524682]).[supplied by OMIM Feb 2011]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.