PrimePCR™ SYBR® Green Assay: HSD11B1L, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0057508

List Price:    $174.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0056403
Assay Design:   exonic
Chromosome Location:   19:5688150-5688363question
Amplicon Length:   184
Splice Variants Targeted:   ENST00000577917 ENST00000581893 ENST00000411793 ENST00000301382 ENST00000581773 ENST00000423665 ENST00000583928 ENST00000342970 ENST00000581521 ENST00000339423

Gene Information

Description Not Available

Gene Symbol:   HSD11B1L
Gene Name:   hydroxysteroid (11-beta) dehydrogenase 1-like
Aliases:   SCDR10, SDR26C2
RefSeq:   NC_000019.9 NG_017015.1 NT_011255.14
Ensembl:   ENSG00000167733
Entrez:   374875
Chromosome Mapping:   19p13.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998000
y-intercept 35.720000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download