PrimePCR™ SYBR® Green Assay: RP11-47I22.4, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best specificity; Same primer pair as used in probe assay qHsaCEP0056221

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: RP11-47I22.4, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: RP11-47I22.4, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0048961
Assay Design:   exonic
Chromosome Location:   14:62124500-62124647question
Amplicon Length:   118
Splice Variants Targeted:   ENST00000354232 ENST00000406053 ENST00000359242 ENST00000294678 ENST00000392385 ENST00000556347 ENST00000551944 ENST00000261200 ENST00000550977 ENST00000409562 ENST00000334567 ENST00000483793 ENST00000558583

Gene Information

Description Not Available

Gene Symbol:   RP11-47I22.4
Gene Name:   Uncharacterized protein
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSG00000258989
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.996100
y-intercept 35.280000
Efficiency 103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download