PrimePCR™ SYBR® Green Assay: CYP2A6, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0056170

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: CYP2A6, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: CYP2A6, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0048910
Assay Design:   exonic
Chromosome Location:   19:41349536-41349627question
Amplicon Length:   62
Splice Variants Targeted:   ENST00000576608 ENST00000564546 ENST00000301141 ENST00000602402 ENST00000283233 ENST00000381031 ENST00000265724 ENST00000602404 ENST00000498737 ENST00000405123 ENST00000185150 ENST00000378239 ENST00000249299 ENST00000361573 ENST00000417961 ENST00000539601 ENST00000541138 ENST00000280758 ENST00000490090 ENST00000314616 ENST00000251289 ENST00000587001 ENST00000309241 ENST00000375943 ENST00000324852 ENST00000450945 ENST00000400962 ENST00000446344 ENST00000278979 ENST00000447647

Gene Information

This gene CYP2A6 encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by phenobarbital. The enzyme is known to hydroxylate coumarin and also metabolizes nicotine aflatoxin B1 nitrosamines and some pharmaceuticals. Individuals with certain allelic variants are said to have a poor metabolizer phenotype meaning they do not efficiently metabolize coumarin or nicotine. This gene is part of a large cluster of cytochrome P450 genes from the CYP2A CYP2B and CYP2F subfamilies on chromosome 19q. The gene was formerly referred to as CYP2A3; however it has been renamed CYP2A6. [provided by RefSeq Jul 2008]

Gene Symbol:   CYP2A6
Gene Name:   cytochrome P450, family 2, subfamily A, polypeptide 6
Aliases:   CPA6, CYP2A, CYP2A3, P450C2A, P450PB
RefSeq:   NC_000019.9 NG_000008.7 NG_008377.1 NT_011109.16
Ensembl:   ENSG00000255974
Entrez:   1548
Chromosome Mapping:   19q13.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.220000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download