PrimePCR™ SYBR® Green Assay: U47924.25, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best efficiency; Same primer pair as used in probe assay qHsaCEP0056008

List Price:    $130.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0048748
Assay Design:   exonic
Chromosome Location:   12:6955862-6955974question
Amplicon Length:   83
Splice Variants Targeted:   ENST00000372462 ENST00000310955 ENST00000216044 ENST00000343949 ENST00000358987 ENST00000538752 ENST00000590798 ENST00000399634 ENST00000406454 ENST00000441525 ENST00000422785 ENST00000362019 ENST00000367737 ENST00000361735 ENST00000367736 ENST00000344419 ENST00000359087 ENST00000290810 ENST00000379463 ENST00000394074 ENST00000259455 ENST00000375807 ENST00000339901 ENST00000534113 ENST00000418703 ENST00000261740 ENST00000541794 ENST00000536838 ENST00000392719 ENST00000554050 ENST00000250373 ENST

Gene Information

Description Not Available

Gene Symbol:   U47924.25
Gene Name:   Uncharacterized protein
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSG00000237240
UniGene:   Not Available
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.380000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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