PrimePCR™ SYBR® Green Assay: SCAND3, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0055978

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: SCAND3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: SCAND3, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0048718
Assay Design:   exonic
Chromosome Location:   6:28554120-28554258question
Amplicon Length:   109
Splice Variants Targeted:   ENST00000383811 ENST00000326775 ENST00000451158 ENST00000394170 ENST00000318217 ENST00000579584 ENST00000583621 ENST00000324306 ENST00000426572 ENST00000370766 ENST00000361328 ENST00000373413 ENST00000322748 ENST00000580306 ENST00000580613 ENST00000454073 ENST00000508341 ENST00000600483 ENST00000575898 ENST00000250076 ENST00000416429 ENST00000377325 ENST00000330236 ENST00000457389 ENST00000536028 ENST00000377294 ENST00000377255 ENST00000252211 ENST00000289788 ENST00000452236 ENST00000426540 ENST

Gene Information

Description Not Available

Gene Symbol:   SCAND3
Gene Name:   SCAN domain containing 3
Aliases:   DKFZp434N092, FLJ31087, KIAA1925, MGC131782, MGC133310, MGC133311, ZFP38-L, ZNF305P2, ZNF452, dJ1186N24.3
RefSeq:   NC_000006.11 NT_007592.15 NT_113891.2
Ensembl:   ENSG00000232040
Entrez:   114821
Chromosome Mapping:   6p22.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.670000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download