PrimePCR™ SYBR® Green Assay: ORM1, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0055966

List Price:    $130.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0048706
Assay Design:   exonic
Chromosome Location:   9:117087073-117087165question
Amplicon Length:   63
Splice Variants Targeted:   ENST00000259396 ENST00000431067 ENST00000412657 ENST00000334564 ENST00000371600 ENST00000522208

Gene Information

This gene encodes a key acute phase plasma protein. Because of its increase due to acute inflammation this protein is classified as an acute-phase reactant. The specific function of this protein has not yet been determined; however it may be involved in aspects of immunosuppression. [provided by RefSeq Jul 2008]

Gene Symbol:   ORM1
Gene Name:   orosomucoid 1
Aliases:   AGP-A, AGP1, ORM
RefSeq:   NC_000009.11 NG_012108.1 NT_008470.19
Ensembl:   ENSG00000229314
Entrez:   5004
UniGene:   Hs.522356
Chromosome Mapping:   9q31-q32

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 37.160000
Efficiency 93

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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