This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCEP0055944
PrimePCR™ PreAmp for SYBR® Green Assay: EXOSC6, Human
PrimePCR™ Template for SYBR® Green Assay: EXOSC6, Human
This gene product constitutes one of the subunits of the multisubunit particle called exosome which mediates mRNA degradation. The composition of human exosome is similar to its yeast counterpart. This protein is homologous to the yeast Mtr3 protein. Its exact function is not known however it has been shown using a cell-free RNA decay system that the exosome is required for rapid degradation of unstable mRNAs containing AU-rich elements (AREs) but not for poly(A) shortening. The exosome does not recognize ARE-containing mRNAs on its own but requires ARE-binding proteins that could interact with the exosome and recruit it to unstable mRNAs thereby promoting their rapid degradation. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.