PrimePCR™ SYBR® Green Assay: CRYZL1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0055790

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: CRYZL1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: CRYZL1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0048530
Assay Design:   exonic
Chromosome Location:   21:34975755-34985852question
Amplicon Length:   68
Splice Variants Targeted:   ENST00000381554 ENST00000290244 ENST00000381540 ENST00000445393 ENST00000361534 ENST00000440526 ENST00000426935 ENST00000431177 ENST00000417979

Gene Information

This gene encodes a protein that has sequence similarity to zeta crystallin also known as quinone oxidoreductase. This zeta crystallin-like protein also contains an NAD(P)H binding site. Alternatively spliced transcript variants have been observed but their full-length nature has not been completely determined. [provided by RefSeq Jul 2008]

Gene Symbol:   CRYZL1
Gene Name:   crystallin, zeta (quinone reductase)-like 1
Aliases:   4P11, DKFZp434M035, QOH-1
RefSeq:   NC_000021.8 NT_011512.11
Ensembl:   ENSG00000205758
Entrez:   9946
Chromosome Mapping:   21q21.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.710000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download