PrimePCR™ SYBR® Green Assay: PRR13, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0055766

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: PRR13, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: PRR13, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0048506
Assay Design:   exonic
Chromosome Location:   12:53837331-53837434question
Amplicon Length:   74
Splice Variants Targeted:   ENST00000335509 ENST00000550785 ENST00000392569 ENST00000541517 ENST00000595360 ENST00000440560 ENST00000375743 ENST00000375733 ENST00000537142 ENST00000248450 ENST00000444053 ENST00000420660 ENST00000429243 ENST00000549924 ENST00000551003 ENST00000549068 ENST00000549740 ENST00000549581 ENST00000547368 ENST00000379786 ENST00000549135 ENST00000541275 ENST00000381683 ENST00000412331 ENST00000406934

Gene Information

Description Not Available

Gene Symbol:   PRR13
Gene Name:   proline rich 13
Aliases:   DKFZp564J157, FLJ23818, FLJ58476, TXR1
RefSeq:   NC_000012.11 NT_029419.12
Ensembl:   ENSG00000205352
Entrez:   54458
Chromosome Mapping:   12q12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.760000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download