PrimePCR™ SYBR® Green Assay: SLC35B4, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0055747

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: SLC35B4, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: SLC35B4, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0048487
Assay Design:   exonic
Chromosome Location:   7:133977337-133977466question
Amplicon Length:   100
Splice Variants Targeted:   ENST00000378509

Gene Information

Glycosyltransferases such as SLC35B4 transport nucleotide sugars from the cytoplasm where they are synthesized to the Golgi apparatus where they are utilized in the synthesis of glycoproteins glycolipids and proteoglycans (Ashikov et al. 2005 [PubMed 15911612]).[supplied by OMIM Mar 2008]

Gene Symbol:   SLC35B4
Gene Name:   solute carrier family 35, member B4
Aliases:   FLJ14697, YEA, YEA4
RefSeq:   NC_000007.13 NT_007933.15
Ensembl:   ENSG00000205060
Entrez:   84912
Chromosome Mapping:   7q33

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.680000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download