PrimePCR™ SYBR® Green Assay: PABPN1L, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0055744

List Price:    $134.00
Your Price:   Log In
Add to Custom Plate

Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0048484
Assay Design:   exonic
Chromosome Location:   16:88930785-88931432question
Amplicon Length:   64
Splice Variants Targeted:   ENST00000594151 ENST00000600603 ENST00000601638 ENST00000599732 ENST00000601675 ENST00000221557 ENST00000391842 ENST00000309880 ENST00000389617 ENST00000498315 ENST00000381848 ENST00000361417 ENST00000356063 ENST00000420323 ENST00000218343 ENST00000397189 ENST00000327797 ENST00000479054 ENST00000345716 ENST00000527990 ENST00000254579 ENST00000423565 ENST00000354475 ENST00000344400 ENST00000223127 ENST00000562374 ENST00000448039 ENST00000297954 ENST00000432730 ENST00000411624 ENST00000395477 ENST

Gene Information

Description Not Available

Gene Symbol:   PABPN1L
Gene Name:   poly(A) binding protein, nuclear 1-like (cytoplasmic)
Aliases:   PABPNL1, ePABP2
RefSeq:   NG_008667.1 NC_000016.9 NT_010542.15
Ensembl:   ENSG00000205022
Entrez:   390748
UniGene:   Hs.730522
Chromosome Mapping:   16q24.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 35.630000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
Get Help

Call us at 1-800-4-BIORAD (1-800-424-6723)

Sign Up for Bio-Rad Updates!
Enter your email address below to receive your choice of the latest news, promotions, and more.