PrimePCR™ SYBR® Green Assay: RP11-274K13.2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0055685

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: RP11-274K13.2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: RP11-274K13.2, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0048425
Assay Design:   exonic
Chromosome Location:   X:135056808-135056920question
Amplicon Length:   83
Splice Variants Targeted:   ENST00000216044 ENST00000551330 ENST00000004980 ENST00000448372 ENST00000339976 ENST00000380367 ENST00000328942 ENST00000381176 ENST00000292431 ENST00000305738 ENST00000376281 ENST00000351839 ENST00000360384 ENST00000457156 ENST00000376264 ENST00000376263 ENST00000371250 ENST00000374553 ENST00000374552 ENST00000563459 ENST00000449606 ENST00000359568 ENST00000541685 ENST00000357337 ENST00000407578 ENST00000544440 ENST00000360084 ENST00000370707 ENST00000275732 ENST00000254029 ENST00000371825 ENST

Gene Information

Description Not Available

Gene Symbol:   RP11-274K13.2
Gene Name:   Novel protein; Uncharacterized protein
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSG00000203945
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 35.860000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download