PrimePCR™ SYBR® Green Assay: FICD, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0055647

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: FICD, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: FICD, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0048387
Assay Design:   exonic
Chromosome Location:   12:108910710-108910841question
Amplicon Length:   102
Splice Variants Targeted:   ENST00000356813 ENST00000371734 ENST00000371732 ENST00000315908 ENST00000344710 ENST00000397525 ENST00000330125 ENST00000397523 ENST00000382180 ENST00000380736 ENST00000303025 ENST00000337414 ENST00000552695 ENST00000552758 ENST00000361549 ENST00000291592 ENST00000307363 ENST00000307377 ENST00000162391 ENST00000428177 ENST00000529708 ENST00000526841 ENST00000600059 ENST00000270238 ENST00000406326

Gene Information

Description Not Available

Gene Symbol:   FICD
Gene Name:   FIC domain containing
Aliases:   HIP13, HYPE, MGC5623, UNQ3041
RefSeq:   NC_000012.11 NT_029419.12
Ensembl:   ENSG00000198855
Entrez:   11153
Chromosome Mapping:   12q24.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.610000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download