PrimePCR™ SYBR® Green Assay: ZNF28, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0055583

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: ZNF28, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: ZNF28, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0048323
Assay Design:   exonic
Chromosome Location:   19:53304795-53304929question
Amplicon Length:   105
Splice Variants Targeted:   ENST00000438150 ENST00000457749 ENST00000391783 ENST00000596559 ENST00000594602 ENST00000339844 ENST00000360272 ENST00000414252 ENST00000595646 ENST00000243639 ENST00000597924 ENST00000396409 ENST00000390651 ENST00000442668 ENST00000545558 ENST00000358855

Gene Information

Description Not Available

Gene Symbol:   ZNF28
Gene Name:   zinc finger protein 28
Aliases:   DKFZp781D0275, KOX24
RefSeq:   NC_000019.9 NT_011109.16
Ensembl:   ENSG00000198538
Entrez:   7576
Chromosome Mapping:   19q

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.994300
y-intercept 38.110000
Efficiency 87

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download