PrimePCR™ SYBR® Green Assay: MIER1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0055544

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: MIER1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: MIER1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0048284
Assay Design:   exonic
Chromosome Location:   1:67423760-67423863question
Amplicon Length:   74
Splice Variants Targeted:   ENST00000357692 ENST00000401041 ENST00000371016 ENST00000371014 ENST00000401042 ENST00000355356 ENST00000371018 ENST00000355977

Gene Information

This gene encodes a protein that was first identified in Xenopus laevis by its role in a mesoderm induction early response (MIER). In human this gene functions as a growth-factor-induced immediate early gene that encodes a transcriptional regulator. Depending on the cellular context this gene can function as both an activator and repressor of gene transcription. Alternative splicing results in multiple isoforms; some of which contain a nuclear localization signal. [provided by RefSeq Aug 2011]

Gene Symbol:   MIER1
Gene Name:   mesoderm induction early response 1 homolog (Xenopus laevis)
Aliases:   DKFZp781G0451, ER1, KIAA1610, MGC131940, MGC150640, MGC150641, MI-ER1, hMI-ER1
RefSeq:   NC_000001.10 NT_032977.9
Ensembl:   ENSG00000198160
Entrez:   57708
Chromosome Mapping:   1p31.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997300
y-intercept 36.410000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download