PrimePCR™ SYBR® Green Assay: ADH4, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0055536

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: ADH4, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: ADH4, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0048276
Assay Design:   exonic
Chromosome Location:   4:100062703-100062797question
Amplicon Length:   65
Splice Variants Targeted:   ENST00000508393 ENST00000265512 ENST00000505590 ENST00000512499 ENST00000504125 ENST00000423445

Gene Information

This gene encodes class II alcohol dehydrogenase 4 pi subunit which is a member of the alcohol dehydrogenase family. Members of this enzyme family metabolize a wide variety of substrates including ethanol retinol other aliphatic alcohols hydroxysteroids and lipid peroxidation products. Class II alcohol dehydrogenase is a homodimer composed of 2 pi subunits. It exhibits a high activity for oxidation of long-chain aliphatic alcohols and aromatic alcohols and is less sensitive to pyrazole. This gene is localized to chromosome 4 in the cluster of alcohol dehydrogenase genes. [provided by RefSeq Jul 2008]

Gene Symbol:   ADH4
Gene Name:   alcohol dehydrogenase 4 (class II), pi polypeptide
Aliases:   ADH-2
RefSeq:   NC_000004.11 NT_016354.19
Ensembl:   ENSG00000198099
Entrez:   127
Chromosome Mapping:   4q21-q24,4q22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.810000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download