PrimePCR™ SYBR® Green Assay: AKAP17A, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0055520

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: AKAP17A, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: AKAP17A, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0048260
Assay Design:   exonic
Chromosome Location:   X:1712533-1712653question
Amplicon Length:   91
Splice Variants Targeted:   ENST00000313871 ENST00000381261

Gene Information

This locus encodes a protein kinase A anchoring protein. The encoded protein is part of the spliceosome complex and is involved in the regulation of alternate splicing in some mRNA precursors. Alternatively spliced transcript variants have been identified for this gene.[provided by RefSeq Sep 2010]

Gene Symbol:   AKAP17A
Gene Name:   A kinase (PRKA) anchor protein 17A
Aliases:   721P, AKAP-17A, CCDC133, CXYorf3, DXYS155E, FLJ98315, MGC125365, MGC125366, MGC39904, PRKA17A, SFRS17A, XE7, XE7Y
RefSeq:   NC_000023.10 NC_000024.9 NG_016337.1 NG_021470.1 NT_167196.1 NT_167204.1
Ensembl:   ENSG00000197976
Entrez:   8227
Chromosome Mapping:   Xp22.33 and Yp11.32

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997900
y-intercept 37.330000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download