PrimePCR™ SYBR® Green Assay: CR1L, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0055484

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: CR1L, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: CR1L, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0048224
Assay Design:   exonic
Chromosome Location:   1:207818524-207850746question
Amplicon Length:   135
Splice Variants Targeted:   ENST00000478953 ENST00000307450 ENST00000597073 ENST00000374461 ENST00000401783 ENST00000407373 ENST00000332585 ENST00000453621 ENST00000431368 ENST00000452656 ENST00000382310 ENST00000437268 ENST00000535268 ENST00000526712 ENST00000427052 ENST00000342160 ENST00000262854 ENST00000296271 ENST00000372980 ENST00000539718 ENST00000317310 ENST00000508064 ENST00000358170 ENST00000354848 ENST00000322918 ENST00000367042 ENST00000367041 ENST00000357714 ENST00000322875 ENST00000367047 ENST00000360212 ENST

Gene Information

Description Not Available

Gene Symbol:   CR1L
Gene Name:   complement component (3b/4b) receptor 1-like
Aliases:   MGC129536
RefSeq:   NC_000001.10 NT_167186.1
Ensembl:   ENSG00000197721
Entrez:   1379
Chromosome Mapping:   1q32.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.996300
y-intercept 36.760000
Efficiency 93

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download