PrimePCR™ SYBR® Green Assay: COL4A6, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0055472

List Price:    $130.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0048212
Assay Design:   exonic
Chromosome Location:   X:107402842-107402939question
Amplicon Length:   68
Splice Variants Targeted:   ENST00000334504 ENST00000372216 ENST00000418180 ENST00000394872 ENST00000545689 ENST00000538570

Gene Information

This gene encodes one of the six subunits of type IV collagen the major structural component of basement membranes. Like the other members of the type IV collagen gene family this gene is organized in a head-to-head conformation with another type IV collagen gene alpha 5 type IV collagen so that the gene pair shares a common promoter. Deletions in the alpha 5 gene that extend into the alpha 6 gene result in diffuse leiomyomatosis accompanying the X-linked Alport syndrome caused by the deletion in the alpha 5 gene. Two splice variants have been identified for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   COL4A6
Gene Name:   collagen, type IV, alpha 6
Aliases:   CXDELq22.3, DELXq22.3, MGC88184
RefSeq:   NG_011977.1 NC_000023.10 NG_012059.1 NT_011651.17
Ensembl:   ENSG00000197565
Entrez:   1288
UniGene:   Hs.145586
Chromosome Mapping:   Xq22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 1.000000
y-intercept 35.800000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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