PrimePCR™ SYBR® Green Assay: GZMM, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0055466

List Price:    $130.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0048206
Assay Design:   exonic
Chromosome Location:   19:549736-549840question
Amplicon Length:   75
Splice Variants Targeted:   ENST00000456197 ENST00000446306 ENST00000355540 ENST00000359863 ENST00000566839 ENST00000570190 ENST00000264553 ENST00000262525 ENST00000254853 ENST00000512825 ENST00000424747 ENST00000328070 ENST00000265310 ENST00000439304 ENST00000308284 ENST00000223210 ENST00000374168 ENST00000374166 ENST00000526792 ENST00000530003 ENST00000531382 ENST00000374162 ENST00000319988 ENST00000565218 ENST00000562259 ENST00000562468 ENST00000561982

Gene Information

Human natural killer (NK) cells and activated lymphocytes express and store a distinct subset of neutral serine proteases together with proteoglycans and other immune effector molecules in large cytoplasmic granules. These serine proteases are collectively termed granzymes and include 4 distinct gene products: granzyme A granzyme B granzyme H and Met-ase also known as granzyme M. [provided by RefSeq Jul 2008]

Gene Symbol:   GZMM
Gene Name:   granzyme M (lymphocyte met-ase 1)
Aliases:   LMET1, MET1
RefSeq:   NC_000019.9 NT_011255.14
Ensembl:   ENSG00000197540
Entrez:   3004
UniGene:   Hs.465511
Chromosome Mapping:   19p13.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997100
y-intercept 36.890000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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