PrimePCR™ SYBR® Green Assay: ZNF772, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0055421

List Price:    $130.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0048161
Assay Design:   exonic
Chromosome Location:   19:57988779-57988893question
Amplicon Length:   85
Splice Variants Targeted:   ENST00000379441 ENST00000551812 ENST00000549884 ENST00000551996 ENST00000179765 ENST00000319080 ENST00000265348 ENST00000535468 ENST00000263208 ENST00000541063 ENST00000546308 ENST00000397256 ENST00000443148 ENST00000383827 ENST00000430793 ENST00000596831 ENST00000600175 ENST00000601768 ENST00000343280 ENST00000427512 ENST00000356584 ENST00000425074 ENST00000325643 ENST00000418034 ENST00000263119 ENST00000405822 ENST00000398319 ENST00000317977 ENST00000284154 ENST00000337755 ENST00000589389 ENST

Gene Information

Description Not Available

Gene Symbol:   ZNF772
Gene Name:   zinc finger protein 772
Aliases:   DKFZp686I1569
RefSeq:   NC_000019.9 NT_011109.16
Ensembl:   ENSG00000197128
Entrez:   400720
UniGene:   Hs.388810
Chromosome Mapping:   19q13.43

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 36.170000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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