PrimePCR™ SYBR® Green Assay: ZNF765, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0055341

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: ZNF765, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: ZNF765, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0048081
Assay Design:   exonic
Chromosome Location:   19:53905437-53911045question
Amplicon Length:   73
Splice Variants Targeted:   ENST00000359798 ENST00000465448 ENST00000486474 ENST00000461321 ENST00000391785 ENST00000301093 ENST00000593941 ENST00000540331 ENST00000602162 ENST00000319783 ENST00000595798 ENST00000601643 ENST00000600943 ENST00000543227 ENST00000540744 ENST00000453741 ENST00000338230 ENST00000595646 ENST00000243639 ENST00000597924 ENST00000396409 ENST00000390651 ENST00000434371 ENST00000549216 ENST00000391777 ENST00000601857 ENST00000595091 ENST00000458035 ENST00000474037 ENST00000467003 ENST00000396408 ENST

Gene Information

Description Not Available

Gene Symbol:   ZNF765
Gene Name:   zinc finger protein 765
Aliases:   DKFZp686J2252
RefSeq:   NC_000019.9 NT_011109.16
Ensembl:   ENSG00000196417
Entrez:   91661
Chromosome Mapping:   19q13.42

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 36.010000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download