PrimePCR™ SYBR® Green Assay: ZNF140, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0055334

List Price:    $130.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0048074
Assay Design:   exonic
Chromosome Location:   12:133659703-133659814question
Amplicon Length:   82
Splice Variants Targeted:   ENST00000355557 ENST00000356456 ENST00000412146 ENST00000440984 ENST00000319849 ENST00000440550 ENST00000429434 ENST00000434248 ENST00000221355 ENST00000598845 ENST00000593605 ENST00000450241 ENST00000414720 ENST00000594395 ENST00000595773 ENST00000599972 ENST00000455521 ENST00000595687 ENST00000340963 ENST00000590503 ENST00000589413 ENST00000352998 ENST00000588371 ENST00000311085 ENST00000539542 ENST00000481138 ENST00000478850 ENST00000427179 ENST00000394226 ENST00000446148 ENST00000274599 ENST

Gene Information

Description Not Available

Gene Symbol:   ZNF140
Gene Name:   zinc finger protein 140
Aliases:   pHZ-39
RefSeq:   NC_000012.11 NT_024477.14
Ensembl:   ENSG00000196387
Entrez:   7699
UniGene:   Hs.181552
Chromosome Mapping:   12q24.33

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997500
y-intercept 36.370000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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