PrimePCR™ SYBR® Green Assay: NCR1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0055296

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: NCR1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: NCR1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0048036
Assay Design:   exonic
Chromosome Location:   19:55418159-55420738question
Amplicon Length:   112
Splice Variants Targeted:   ENST00000339591 ENST00000449880 ENST00000450894 ENST00000200181 ENST00000579662 ENST00000397786 ENST00000253063 ENST00000310123 ENST00000392225 ENST00000262919 ENST00000291890 ENST00000598576 ENST00000594765 ENST00000350790 ENST00000338835 ENST00000357397 ENST00000447255 ENST00000267082 ENST00000551319 ENST00000422257 ENST00000550743 ENST00000338737 ENST00000590071 ENST00000371505 ENST00000571635 ENST00000570317 ENST00000598228 ENST00000602031 ENST00000576385 ENST00000576881 ENST00000571087 ENST

Gene Information

Description Not Available

Gene Symbol:   NCR1
Gene Name:   natural cytotoxicity triggering receptor 1
Aliases:   CD335, FLJ99094, LY94, NK-p46, NKP46
RefSeq:   NC_000019.9 NT_011109.16
Ensembl:   ENSG00000189430
Entrez:   9437
Chromosome Mapping:   19q13.42

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.960000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download