PrimePCR™ SYBR® Green Assay: PCDH18, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0055285

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: PCDH18, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: PCDH18, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0048025
Assay Design:   exonic
Chromosome Location:   4:138449700-138449795question
Amplicon Length:   66
Splice Variants Targeted:   ENST00000412923 ENST00000344876 ENST00000507846 ENST00000511115 ENST00000510305

Gene Information

This gene belongs to the protocadherin gene family a subfamily of the cadherin superfamily. This gene encodes a protein which contains 6 extracellular cadherin domains a transmembrane domain and a cytoplasmic tail differing from those of the classical cadherins. Although its specific function is undetermined the cadherin-related neuronal receptor is thought to play a role in the establishment and function of specific cell-cell connections in the brain. [provided by RefSeq Jul 2008]

Gene Symbol:   PCDH18
Gene Name:   protocadherin 18
Aliases:   DKFZp434B0923, KIAA1562, PCDH68L
RefSeq:   NC_000004.11 NT_016354.19
Ensembl:   ENSG00000189184
Entrez:   54510
Chromosome Mapping:   4q31

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 35.660000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download