PrimePCR™ SYBR® Green Assay: KCTD21, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0055272

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: KCTD21, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: KCTD21, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0048012
Assay Design:   exonic
Chromosome Location:   11:77885488-77885577question
Amplicon Length:   60
Splice Variants Targeted:   ENST00000340067 ENST00000526208 ENST00000525447 ENST00000529350 ENST00000530018 ENST00000528776 ENST00000338754 ENST00000353479 ENST00000369733 ENST00000340001 ENST00000453985 ENST00000370732 ENST00000409671 ENST00000566264 ENST00000369135 ENST00000460099 ENST00000352832 ENST00000425826 ENST00000366552 ENST00000581312

Gene Information

Description Not Available

Gene Symbol:   KCTD21
Gene Name:   potassium channel tetramerisation domain containing 21
Aliases:   Not Available
RefSeq:   NC_000011.9 NT_167190.1
Ensembl:   ENSG00000188997
Entrez:   283219
Chromosome Mapping:   11q14.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 36.370000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download