This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Recommended - best efficiency; Same primer pair as used in probe assay qHsaCEP0055264
PrimePCR™ PreAmp for SYBR® Green Assay: LRRK2, Human
PrimePCR™ Template for SYBR® Green Assay: LRRK2, Human
This gene is a member of the leucine-rich repeat kinase family and encodes a protein with an ankryin repeat region a leucine-rich repeat (LRR) domain a kinase domain a DFG-like motif a RAS domain a GTPase domain a MLK-like domain and a WD40 domain. The protein is present largely in the cytoplasm but also associates with the mitochondrial outer membrane. Mutations in this gene have been associated with Parkinson disease-8. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.