PrimePCR™ SYBR® Green Assay: SNTN, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0055261

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: SNTN, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: SNTN, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0048001
Assay Design:   exonic
Chromosome Location:   3:63638359-63638458question
Amplicon Length:   70
Splice Variants Targeted:   ENST00000374572 ENST00000552779 ENST00000550330 ENST00000383198 ENST00000550915 ENST00000549142 ENST00000379445 ENST00000395322 ENST00000343837 ENST00000469440 ENST00000496807 ENST00000434618 ENST00000456592 ENST00000417059 ENST00000549949 ENST00000553235 ENST00000383066 ENST00000551943 ENST00000546700 ENST00000249330 ENST00000445482 ENST00000348628 ENST00000533760 ENST00000401611 ENST00000316851 ENST00000416701 ENST00000591454 ENST00000593059 ENST00000591218 ENST00000590031 ENST00000576604 ENST

Gene Information

Description Not Available

Gene Symbol:   SNTN
Gene Name:   sentan, cilia apical structure protein
Aliases:   FLJ44379, S100A1L, S100AL, sentan
RefSeq:   NC_000003.11 NT_022517.18
Ensembl:   ENSG00000188817
Entrez:   132203
Chromosome Mapping:   3p14.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.580000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download