PrimePCR™ SYBR® Green Assay: COL25A1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0055240

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: COL25A1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: COL25A1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0047980
Assay Design:   exonic
Chromosome Location:   4:110223130-110223229question
Amplicon Length:   70
Splice Variants Targeted:   ENST00000455752 ENST00000320574 ENST00000564535 ENST00000568089 ENST00000393847 ENST00000572888 ENST00000412757 ENST00000597002 ENST00000399132 ENST00000399127 ENST00000399126 ENST00000505591 ENST00000392401 ENST00000416392 ENST00000447252 ENST00000534366 ENST00000528919 ENST00000398656 ENST00000326134 ENST00000544576 ENST00000396976 ENST00000562072 ENST00000568898 ENST00000396972 ENST00000344113 ENST00000554584 ENST00000555002 ENST00000394768 ENST00000358025 ENST00000357395 ENST00000328194 ENST

Gene Information

This gene encodes a brain-specific membrane associated collagen. A product of proteolytic processing of the encoded protein CLAC (collagenous Alzheimer amyloid plaque component) binds to amyloid beta-peptides found in Alzheimer amyloid plaques but CLAC inhibits rather than facilitates amyloid fibril elongation (PMID: 16300410). A study of over-expression of this collagen in mice however found changes in pathology and behavior suggesting that the encoded protein may promote amyloid plaque formation (PMID: 19548013). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Dec 2011]

Gene Symbol:   COL25A1
Gene Name:   collagen, type XXV, alpha 1
Aliases:   CLAC, CLACP
RefSeq:   NC_000004.11 NT_016354.19
Ensembl:   ENSG00000188517
Entrez:   84570
Chromosome Mapping:   4q25

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 36.090000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download