PrimePCR™ SYBR® Green Assay: HES4, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0055224

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: HES4, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: HES4, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0047964
Assay Design:   exonic
Chromosome Location:   1:935085-935190question
Amplicon Length:   76
Splice Variants Targeted:   ENST00000371833 ENST00000382882 ENST00000416258 ENST00000411638 ENST00000542778 ENST00000374103 ENST00000419399 ENST00000432695 ENST00000311620 ENST00000431565 ENST00000325404 ENST00000378295 ENST00000378288 ENST00000357733 ENST00000346387 ENST00000377748 ENST00000377725 ENST00000377728 ENST00000377732 ENST00000400915 ENST00000377740 ENST00000537245 ENST00000535355 ENST00000340850 ENST00000400913 ENST00000377737 ENST00000544978 ENST00000407192 ENST00000420329 ENST00000268184 ENST00000322342 ENST

Gene Information

Description Not Available

Gene Symbol:   HES4
Gene Name:   hairy and enhancer of split 4 (Drosophila)
Aliases:   bHLHb42
RefSeq:   NC_000001.10 NT_004350.19
Ensembl:   ENSG00000188290
Entrez:   57801
Chromosome Mapping:   1p36.33

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 37.060000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download