PrimePCR™ SYBR® Green Assay: ZCCHC13, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0055201

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: ZCCHC13, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: ZCCHC13, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0047941
Assay Design:   exonic
Chromosome Location:   X:73524210-73524314question
Amplicon Length:   75
Splice Variants Targeted:   ENST00000339534

Gene Information

This gene appears to represent an intronless retrocopy of a related multi-exon gene located on chromosome 3. However the CDS of this intronless gene remains relatively intact it is conserved in other mammalian species it is known to be transcribed and it is therefore thought to encode a functional protein. The encoded protein contains six CCHC-type zinc fingers and is thus thought to function as a transcription factor. [provided by RefSeq May 2010]

Gene Symbol:   ZCCHC13
Gene Name:   zinc finger, CCHC domain containing 13
Aliases:   CNBP2, ZNF9L
RefSeq:   NC_000023.10 NG_021228.1 NT_011669.17
Ensembl:   ENSG00000187969
Entrez:   389874
Chromosome Mapping:   Xq13.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 36.150000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download