This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Recommended - best efficiency; Same primer pair as used in probe assay qHsaCEP0055198
PrimePCR™ PreAmp for SYBR® Green Assay: COL14A1, Human
PrimePCR™ Template for SYBR® Green Assay: COL14A1, Human
Type XIV collagen is a fibril-associated collagen with an interrupted triple helix. It interacts with the fibril surface and regulates fibrillogenesis (Ansorge et al. 2009 [PubMed 19136672]).[supplied by OMIM Aug 2010]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.