PrimePCR™ SYBR® Green Assay: KRT12, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0055161

List Price:    $130.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0047901
Assay Design:   exonic
Chromosome Location:   17:39023063-39023200question
Amplicon Length:   108
Splice Variants Targeted:   ENST00000356003 ENST00000448661 ENST00000359154 ENST00000357195 ENST00000336498 ENST00000507661 ENST00000414545 ENST00000400274 ENST00000457905 ENST00000466639 ENST00000435722 ENST00000417564 ENST00000318711 ENST00000427143 ENST00000429591 ENST00000301067 ENST00000269576 ENST00000251643 ENST00000359428 ENST00000411637 ENST00000483497 ENST00000451030 ENST00000572470

Gene Information

KRT12 encodes the type I intermediate filament chain keratin 12 expressed in corneal epithelia. Mutations in this gene lead to Meesmann corneal dystrophy. [provided by RefSeq Jul 2008]

Gene Symbol:   KRT12
Gene Name:   keratin 12
Aliases:   K12
RefSeq:   NC_000017.10 NG_008077.1 NT_010783.15
Ensembl:   ENSG00000187242
Entrez:   3859
UniGene:   Hs.66739
Chromosome Mapping:   17q12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998900
y-intercept 35.560000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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