PrimePCR™ SYBR® Green Assay: NAP1L1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0055149

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: NAP1L1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: NAP1L1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0047889
Assay Design:   exonic
Chromosome Location:   12:76453918-76454044question
Amplicon Length:   97
Splice Variants Targeted:   ENST00000261182 ENST00000552056 ENST00000393263 ENST00000431879 ENST00000547773 ENST00000544816 ENST00000542344 ENST00000535020 ENST00000549596 ENST00000552342 ENST00000548044 ENST00000550934 ENST00000551992 ENST00000548273 ENST00000551600 ENST00000547704 ENST00000551524 ENST00000547479

Gene Information

This gene encodes a member of the nucleosome assembly protein (NAP) family. This protein participates in DNA replication and may play a role in modulating chromatin formation and contribute to the regulation of cell proliferation. Alternative splicing of this gene results in several transcript variants; however not all have been fully described. [provided by RefSeq Jul 2008]

Gene Symbol:   NAP1L1
Gene Name:   nucleosome assembly protein 1-like 1
Aliases:   FLJ16112, MGC23410, MGC8688, NAP1, NAP1L, NRP
RefSeq:   NC_000012.11 NT_029419.12
Ensembl:   ENSG00000187109
Entrez:   4673
Chromosome Mapping:   12q21.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.810000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download